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Modified Nucleosides & Custom Oligonucleotides

Synthetic Services by Polaris Oligonucleotides

Unlock the Power of Precision with Polaris Oligonucleotides

The need for customized and chemically modified oligonucleotides is increasing in the fields of molecular biology and biomedicines. Polaris Oligonucleotides stands at the forefront of this innovation, offering unparalleled expertise in synthesizing modified nucleotides. We offer custom synthetic services for oligonucleotides (oligoribonucleotides, polydeoxyribonucleotides, modified nucleotides.)

We can handle challenging syntheses and deliver quality results no matter the project’s complexity. Our goal is to meet your specifications and exceed your expectations.

1. DNA primers, tags, and specialty products

Fast and efficient service for custom synthesis of DNA primers for multiple applications.

DNA-encoded library supplies: custom primers and tags collections. All deliverable provided as sodium salt to insure the efficiency of downstream enzyme-mediated reactions.

2. Chemically Modified Oligonucleotides

Custom Modifications: Tailor-made oligonucleotides with specific chemical modifications to suit your research needs.

High Purity: Achieve superior performance with our high-purity oligonucleotides, ensuring reliable and reproducible results.

Wide Range of Modifications: From fluorescent labels and quenchers to phosphate and ribose modifications, we offer a broad spectrum of custom chemical modifications to enhance your experiments. Can’t find the modification you need in our catalog? Contact us to learn more about our custom phosphonamidite development services.

 

3. Oligoribonucleotides

 

RNA Synthesis: Expertise in the synthesis of RNA oligonucleotides, including complex modifications for advanced applications.

Stable and Reliable: Our modified oligoribonucleotides are designed and formulated for maximal

stability in various experimental conditions.

Innovative Solutions: Access cutting-edge solutions for RNA interference, CRISPR, and other RNA-based technologies.

4. Difficult and Delicate Syntheses

 

Challenging Projects: Specialized in handling complex and delicate synthetic projects that require precision and expertise.

Customized Approaches: Work with our team to develop custom synthesis strategies tailored to your unique requirements.

Expert Support: Receive dedicated support from our team of experts throughout your project, from conception to completion.

Purity and Quality: We pride ourselves in the unmatched quality of our deliverables and offer multiple purification options to best match your research needs.

Applications of Modified Nucleotides

Modified nucleotides are essential tools in numerous areas of molecular biology and biotechnology. Their applications are vast and varied, including:

 

1. Molecular Diagnostics

 

Probe Design: Modified nucleotides are used to create probes for detecting specific DNA or RNA sequences in diagnostic assays.

qPCR: Enhance the sensitivity and specificity of quantitative PCR (qPCR) assays with custom-modified nucleotides.

Microarrays: Improve the performance of microarray technologies with high-affinity modified oligonucleotides with improved stability.

 

2. Therapeutic Applications

 

Therapeutic Oligos: Use modified oligonucleotides for the development of therapies to treat cancer and genetic disorders. Take advantage of the high quality and conformity and guaranteed reproducibility of our deliverables.

RNAi (RNA-interference), siRNA (short interfering RNA), and ASO (antisense oligonucleotides): Enhance the stability and efficacy of RNAi-based therapeutics (ASO, siRNA) through systematic residue modification. Protect the molecules against degradation by nuclease while improving upon its pharmacokinetic properties.

 

3. Research and Development

 

Protein-Nucleic Acid Interactions: Study protein-nucleic acid interactions using nucleotides modified with specific chemical mediators. Large array of available Photo crosslinkers targeting various residues (ex. Serine, Cysteine, Lysines, Tyrosines).

Synthetic Biology: Advance synthetic biology projects with custom-designed nucleotides for building complex systems. Take advantage of our multiple options for 5’- and 3’-labeling, phosphorylation, and conjugations (including oligonucleotides-drug conjugates and oligonucleotides-peptides conjugates).

 

System biology: Probe biological systems with tailor-made oligonucleotides sensors.

Advanced Customization Options

Polaris Oligonucleotides offers a wide array of customization options to meet the unique needs of your research. Our advanced customization services include:

1. Labeling and Detection

 

Fluorescent Labels: Incorporate fluorescent dyes for real-time visualization and tracking of nucleic acids. Multiple options for excitation and emission wavelengths.

 

Quenchers: Use quenchers for designing molecular beacons and other fluorescence-based assays.

 

Biotinylation: Add biotin for streptavidin-based isolation and purification methods.

2. Backbone Modifications

Phosphorothioate Bonds: Increase the nuclease resistance of oligonucleotides with phosphorothioate modifications.

Locked Nucleic Acids (LNAs): Incorporate LNAs for duplex stabilization by hybridization enhancements. Improves resistance to nucleases. Peptide Nucleic Acids (PNAs): Non-nucleoside option for duplex stabilization. While completely inert to nucleases, PNA residues cannot be amplified by PCR nor used for RISK-based RNAi. Phosphonodiamidite Morpholino Oligomers (PMOs): Confers biological resistance. Used extensively in siRNA and other oligonucleotides splicing modulators.

 

3. Base Modifications

 

5-Methylcytosine: Mimic natural DNA methylation with 5-methylcytosine modifications.

Inosine: Incorporate inosine for improved base pairing flexibility in hybridization assays.

Pseudouridine: Enhance the stability and functionality of RNA with pseudouridine modifications.

Deoxy-Uridine: Introduce a convenient enzymatic cleavage site (USERTM enzyme mix) in DNA oligomers.

5-nitroindole: Unnatural base with universal base-pairing properties.

 

4. Spacers and Linkers

Spacers and linkers: Multiple alkyl and PEG spacer lengths available.

At Polaris Oligonucleotides, we believe in a collaborative approach to custom synthesis. Our process involves close communication and partnership with our clients to ensure that every detail of the project is meticulously planned and executed. Here’s how we work with you:

1. Consultation and Planning

Initial Consultation: Discuss your project requirements and objectives with our expert team.

Feasibility Assessment: Evaluate the feasibility of your custom synthesis project and provide recommendations.

Detailed Proposal: A detailed proposal outlining the synthesis strategy, timeline, and cost, will be provided within 24 hours following this initial complementary consultation.

 

2. Custom Synthesis Process

 

Design and Synthesis: Design and synthesize the modified nucleotides according to your specifications.

Purifications and Quality Control: Perform stringent purifications procedures to obtain reach the desired levels of purity and conduct rigorous quality control measures to ensure the integrity and performance of the synthesized products.

Progress Updates: Provide regular updates on the progress of your project and address any questions or concerns.

3. Delivery and Support

Timely Delivery: Ensure prompt delivery of your custom-synthesized nucleotides. While each project’s timeline will be different, a detailed estimate will be provided beforehand and updated as needed.

Post-Delivery Support: Offer ongoing support and consultation to help you integrate the synthesized nucleotides into your research and provide tips for storage. Feedback and Improvement: Gather feedback to continuously improve our services and meet your evolving needs.

Are you ready to elevate your research with custom synthetic services for modified nucleotides? Contact Polaris Oligonucleotides today to get a quote. Our team is eager to discuss your project and provide you with the high-quality, customized oligonucleotides you need to achieve your scientific goals.

 

Unlock the full potential of your research with Polaris Oligonucleotides – your trusted partner in custom nucleotide synthesis.

Future Trends in Modified Nucleotides

The field of modified nucleotides is continuously evolving, driven by advancements in technology and growing research demands. Here are some emerging trends that are shaping the future of this field:

 

1. Enhanced Gene Editing Technologies

 

Precision Editing: The development of more precise and efficient gene editing technologies, such as CRISPR-Cas variants, is increasing the demand for highly specialized modified nucleotides. Gene editing technologies are increasingly used in molecular biology research and therapeutic applications are starting to appear. The use of gene editing for therapeutic purposes is expected to grow, with modified nucleotides playing a critical role in ensuring the accuracy, efficacy, and stability of the final therapy.

1. RNA-Based Therapeutics

mRNA Vaccines: The success of mRNA vaccines for COVID-19 has highlighted the potential of RNA-based therapeutics.

RNA Interference: The therapeutic use of RNA interference (RNAi) and small interfering RNA (siRNA) continues to expand, necessitating customized and modified nucleotides for optimal performance.

Modified oligonucleotides are used in a wide array of RNA-based medicines, to help improve the efficacy and biological resiliency of the medicine.

1. Advanced Diagnostic Tools

Liquid Biopsies: Modified nucleotides are being used to develop liquid biopsy technologies that allow for non-invasive detection and monitoring of diseases such as cancer.

Point-of-Care Testing: The demand for rapid and accurate point-of-care diagnostic tests is increasing, driving the need for high-performance modified nucleotides. Reproducibility and stability in time of the oligonucleotides are two key elements for their successful use in biosensing applications.

1. Synthetic Biology Innovations

Genetic Circuits: Advances in synthetic biology are enabling the design of complex genetic circuits, requiring precise synthetic procedures to achieve the desired arrangement and functions. These may incorporate modified residues to modulate duplex stability, or to include other molecular effectors.

Bioengineering Applications: Modified nucleotides are being used to engineer novel biological systems with applications in biotechnology, agriculture, and environmental science.

Partnering with Polaris Oligonucleotides

Partnering with Polaris Oligonucleotides means more than just accessing high-quality products—it means joining a community dedicated to scientific excellence and innovation. Here’s what you can expect when you partner with us:

1. Tailored Solutions

Custom Synthesis: Receive oligonucleotides that are precisely tailored to your research needs, ensuring optimal performance and reliability.

Collaborative Approach: Work closely with our team to develop custom solutions that address your specific challenges and goals, no matter how complex they are.

1. Exceptional Quality

Rigorous Standards: Benefit from our commitment to the highest standards of quality and

precision in every aspect of our synthesis process.

Consistent Results: Achieve consistent and reproducible results with our meticulously synthesized nucleotides and be confident in your results thanks to our meticulous quality control process.

1. Comprehensive Support

Expert Guidance: Access expert guidance and support throughout your project, from initial consultation to post-delivery assistance.

Educational Resources: Stay informed and up-to-date with our extensive range of educational resources and technical support materials.

1. Innovation and Advancement

Cutting-Edge Techniques: Leverage the latest advancements in nucleotide synthesis and modification to stay at the forefront of your field.

Fully customizable service: Take advantage of our team’s large chemistry expertise to design non-commercial monomers and novel nonnatural residues.

Research Collaboration: Explore opportunities for research collaboration and joint projects to drive scientific discovery and innovation.

How to Get Started

Getting started with Polaris Oligonucleotides is simple and straightforward. Here’s how you can begin your journey with us:

1. Contact Us

Reach Out: Contact us via phone, email, or through our website to discuss your project and requirements.

Initial Consultation: Schedule an initial, free consultation with our expert team to explore your needs and objectives.

1. Project Proposal

Detailed Proposal: Receive a detailed project proposal outlining the synthesis strategy, timeline, and cost within 24h of the orientation consultation.

Review and Approve: Review the proposal and provide feedback or approval to proceed with the project.

 

1. Synthesis and Quality Control

 

Custom Synthesis: Our team will design and synthesize the modified nucleotides according to your specifications.

Rigorous Testing: Conduct rigorous quality control measures to ensure the highest standards of purity and performance.

1. Delivery and Support

Timely Delivery: Receive your custom-synthesized nucleotides promptly and efficiently.

 

Ongoing Support: Access ongoing support and consultation to help you integrate the nucleotides into your research and achieve your goals.

Are you ready to take your research to the next level with custom synthetic services for modified nucleotides? Contact Polaris Oligonucleotides today to get a quote and start your journey towards scientific excellence. Our team is here to support you every step of the way. Unlock the full potential of your research with Polaris Oligonucleotides – your trusted partner in custom nucleotide synthesis.

 

By focusing on precision, quality, and customer satisfaction, Polaris Oligonucleotides aims to be the leading provider of custom synthetic services for modified nucleotides. Whether you are in academia, biotech, or pharmaceuticals, our expertise and dedication will help you achieve your research objectives with confidence.

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