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Instructions
Order Information
Wobble Bases
Additional Services
Order_Information
Order Information
General: Enter all sequences 5' to 3', starting with the character "5"
Item | Enter | Example | Description |
|---|---|---|---|
DNA | Enter bases (A,T,C,G) | 5ATCGCTAG | 5'-ATCGCTAG-3' |
RNA | Enter bases in paranthesis with "r" prefix r(A, U, T, G) | 5r(UUUACCGA) | RNA, UUUACCGA |
Mixed DNA-RNA | Enter DNA bases normally, and RNA bases in paranthesis with 'r" prefix | 5ACTGCGT(rU)C(rG) | DNA+RNA, ACTGCGTUCG |
LNA | Enter bases in parenthesis with "+" prefix | 5ACT(+G)(+G)(+T)CG | DNA+LNA, ACTGGTCG |
PMO | Enter bases in parenthesis with "m" prefix | 5(mC)GTGCA(mC)(mC) | DNA+PMO, CGTGCACC |
Degenarate bases | Use UIPAC nomenclature (see wobble bases) | 5ACTTGCNN | DNA with two degenerate bases (3'), ACTTGCNN |
Internal modifications (see Modifications tab) | Enter the modified base followed by the modification code in brackets | 5r(UUCGU[Biotin]CU) | RNA with internal biotinylated U, UUCGUCU |
Terminal modifications (see modifications tab) | Used the dropdopwn menu for 5' and 3' modifications | /5Phos/AAATGCC/3Biotin/ | DNA with 5' Phosphate and 3' Biotin, Phos-AAATGCC-Biotin |
User supplied modifications (See User-Suppplied nt tab) | Enter user-supplied bases as (#1), (#2), (#3), etc... (with each defined in the appropriate tab) | 5CCTG(#1)C(#2)G | DNA containing two user-supplied bases, CCTG(User supplied 1)C(Use-supplied 2)G |
Phosphonothioates | Entre "*" to denote phosphonothioate linkages | 5CT*G*GC*G | DNA with 3 phosphonothioates linkages, CT-G-GC-G |
Purificaton
Purification
Name | Description |
|---|---|
Desalting (Desalt) | Removal of excess salts (no purity guarantee) |
Ion-pairing HPLC (IPHPLC) | Ion-pairing reverse phase high pressure liquid chromatography |
Anion exchange HPLC (AXHPLC) | Anion-exchange chromatography |
Dual HPLC (dHPLC) | AX-HPLC followed by IP-HPLC |
Polyacrylamide gel electrophoresis (PAGE) | Preparative PAGE (Note: PAGE purification leaves behind polyacrylamide residues. PAGE + IP-HPLC recommended for sensitive applications) |
PAGE + Ion-pairing IPHPLC (PAGE+IPHPLC) | Preparative PAGE followed by IP-HPLC (removes polyacrylamide contamination) |
Wobble_Bases
Wobble Bases
Note: Oligonucleotides containing degenerate bases cannot be evaluated by mass-spectrommetry
Wobble | One letter code | Description |
|---|---|---|
2 nt wobble | R | A+G |
2 nt wobble | W | A+T |
2 nt wobble | M | A+C |
2 nt wobble | Y | C+T |
2 nt wobble | S | C+G |
2 nt wobble | K | G+T |
3 nt wobble | B | C+T+G |
3 nt wobble | D | A+G+T |
3 nt wobble | H | A+C+T |
3 nt wobble | V | A+C+G |
Universal wobble | N | A+C+G+T |
Additonal_Services
Additional Services
Name | Description |
|---|---|
Sodium exchange (NaEx) | Exchange phospates counter-ions to sodium (free) |
Additionnal IP-HPLC analysis | Analysis by IP-HPLC or HILIC-HPLC |
PAGE analysis | Oligo analysis by denaturing polyacrylamide gel electrophoresis |
Gel electrophoresis analysis | Oligo analysis by agarose gel electrophoresis |
Hybridization | Duplex formation from two complementary sequences |
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